Molecular Biology
- 10cm and 15 cm plate grids for colony PCR
- Colony PCR protocol (using Phusion polymerase)
- Colony PCR protocol (using homemade Taq/Pfu polymerase)
- Electrocompetent E. coli preparation
- Ethanol precipitation of DNA
- Gateway cloning pipeline
- PEG/DMSO competent cells
- qPCR (using BioRad Sso polymerase)
- qPCR primer validation
- RNA purification
- Yeast colony PCR