Methods and Protocols
Inspired by Michael Koelle's incredibly helpful C. elegans protocol page, I've provided some of the protocols that I routinely use in my research and reliably work for me. I've tried to credit the source of the protocol, where appropriate, and apologize for any omissions. Recently, I've developed an improved method to knock epitopes into the genome using CRISPR/Cas9. Co-selection for repair of a temperature-sensitive allele of pha-1 combined with inactivation of non-homologous end-joining results in highly efficient knock-in at other genomic loci. Comments and feedback are very welcome and can be emailed to jward2@ucsc.edu.
